Journal: Micromachines
Article Title: Portable Fluorescence Microarray Reader-Enabled Biomarker Panel Detection System for Point-of-Care Diagnosis of Lupus Nephritis
doi: 10.3390/mi16020156
Figure Lengend Snippet: ( A ) Layout of the biomarker microarray (BMA) slide, showing the arrangement of capture antibodies and controls within each well. ( B ) Illustration of the BMA slide design with 16 wells and its alignment within the slide holder for analysis. The numbers along the side correspond to the row numbers of each well, while the numbers along the bottom represent the column positions of the slide.
Article Snippet: The slides were prepared using a non-contact microarray printing robot (sciFLEXARRAYER S3; Scienion GmbH, Berlin, Germany), which printed capture antibodies for each biomarker in triplicate at a controlled drop volume of 450 ± 20 pL.
Techniques: Biomarker Assay, Microarray
Figure S7 ). Binding efficiency is based on the relative on-rate of IgG to immobilized GPCs and is indicated as follows: +++, very strong binding; ++ strong binding; +, moderate binding; −, minimal binding. Proposed IgG stoichiometry per GPC is estimated based on relative R max values under the assumption that the highest R max indicates full occupancy and that 37.7H has a preferred occupancy of 3 Fabs per trimer, as in the crystal structure. (B) mAb neutralization of pseudoviruses derived from LASV LIV (strain Josiah), LII (strain NIG08-A41), and LIII (strain CSF). Dotted lines indicate 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates. (C) Thermostability of LIV GPC-I53-50A in complex with indicated Fabs assessed by nanoDSF. Points represent the T m of each complex. Each melting curve is a representative of triplicate curves with T m within ±0.1°C. (D) Synthetic matriglycan competition microarray measuring StrepTagged GPC-I53-50A binding to matriglycan with and without pretreatment with 12.1F and 19.7E IgG. GPC-I53-50A bound to matriglycan was detected using StrepMAB Ab (
Figure S9 E). Column height reflects the mean RFU with error bars indicating standard deviation. Statistical differences between the groups (n = 4 technical replicates) were determined using two-tailed Mann-Whitney U tests ( ∗ p < 0.05). (E) BLI competition analysis of immobilized GPC bound to indicated IgG and then exposed to recombinant LAMP-1 at a pH of 5 (
Figure S9 F). Presented data indicate representative curves from three technical replicates. " width="100%" height="100%">
Journal: Cell Reports
Article Title: Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies
doi: 10.1016/j.celrep.2023.112524
Figure Lengend Snippet: Characterization of the neutralizing GP1-A-specific mAbs 12.1F and 19.7E (A) Summary of mAb binding to GPCs by BLI (raw data in Figure S7 ). Binding efficiency is based on the relative on-rate of IgG to immobilized GPCs and is indicated as follows: +++, very strong binding; ++ strong binding; +, moderate binding; −, minimal binding. Proposed IgG stoichiometry per GPC is estimated based on relative R max values under the assumption that the highest R max indicates full occupancy and that 37.7H has a preferred occupancy of 3 Fabs per trimer, as in the crystal structure. (B) mAb neutralization of pseudoviruses derived from LASV LIV (strain Josiah), LII (strain NIG08-A41), and LIII (strain CSF). Dotted lines indicate 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates. (C) Thermostability of LIV GPC-I53-50A in complex with indicated Fabs assessed by nanoDSF. Points represent the T m of each complex. Each melting curve is a representative of triplicate curves with T m within ±0.1°C. (D) Synthetic matriglycan competition microarray measuring StrepTagged GPC-I53-50A binding to matriglycan with and without pretreatment with 12.1F and 19.7E IgG. GPC-I53-50A bound to matriglycan was detected using StrepMAB Ab ( Figure S9 E). Column height reflects the mean RFU with error bars indicating standard deviation. Statistical differences between the groups (n = 4 technical replicates) were determined using two-tailed Mann-Whitney U tests ( ∗ p < 0.05). (E) BLI competition analysis of immobilized GPC bound to indicated IgG and then exposed to recombinant LAMP-1 at a pH of 5 ( Figure S9 F). Presented data indicate representative curves from three technical replicates.
Article Snippet: sciFLEXARRAYER S3 non-contact microarray , Scienion Inc. , N/A.
Techniques: Binding Assay, Neutralization, Derivative Assay, Nano Differential Scanning Fluorimetry, Microarray, Standard Deviation, Two Tailed Test, MANN-WHITNEY, Recombinant
Journal: Cell Reports
Article Title: Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies
doi: 10.1016/j.celrep.2023.112524
Figure Lengend Snippet: Isolation of a mAb using GPC-I53-50A (A) BLI sensorgrams depicting immobilized GPC-I53-50A binding to S370.7 IgG in a dose-dependent manner. IgG concentrations used were 50, 25, and 12.5 nM. K D value determined using a 1:1 binding profile and assuming partial dissociation. Further details in A. (B) LIV LASV pseudovirus neutralization by S370.7. Dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates. (C) BLI sensorgram comparing immobilized GPC binding by S370.7 IgG and Fab. IgG and Fab were added at an equimolar concentration of 400 nM. Presented data indicate representative curves from three technical replicates. (D) Thermostability of LIV GPC-I53-50A in complex with S370.7 assessed by nanoDSF. Points represent the T m . Each melting curve is a representative of triplicate curves with T m within ±0.1°C. (E) Synthetic matriglycan binding microarray of StrepTagged GPC-I53-50A bound to S370.7 IgG and detected using StrepMAB Ab. Column height reflects the mean with error bars indicating standard deviation. Statistical differences between the groups (n = 4 technical replicates) were determined using two-tailed Mann-Whitney U tests ( ∗ p < 0.05). (F) BLI analysis of immobilized GPC bound to S370.7 or 25.10C IgG and then exposed to recombinant LAMP-1 at a pH of 5. Presented data indicate representative curves from three technical replicates.
Article Snippet: sciFLEXARRAYER S3 non-contact microarray , Scienion Inc. , N/A.
Techniques: Isolation, Binding Assay, Neutralization, Concentration Assay, Nano Differential Scanning Fluorimetry, Microarray, Standard Deviation, Two Tailed Test, MANN-WHITNEY, Recombinant
Journal: Cell Reports
Article Title: Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies
doi: 10.1016/j.celrep.2023.112524
Figure Lengend Snippet:
Article Snippet: sciFLEXARRAYER S3 non-contact microarray , Scienion Inc. , N/A.
Techniques: Virus, Recombinant, Electron Microscopy, Mass Spectrometry, Sequencing, Lysis, Transfection, Luciferase, Plasmid Preparation, Software, Single-cell Analysis, Mutagenesis, Ligation, Nano Differential Scanning Fluorimetry, Expressing, Staining, Amplification, Inverted Microscopy, Microscopy, Clinical Proteomics, Microarray
